Overview

STEMdiff™ Branching Lung Organoid Kit (Catalog #100-0195) is a serum-free medium system for efficient and reproducible generation of branching lung organoids from human embryonic stem (ES) and induced pluripotent stem (iPS) cells through four stages of differentiation: 1) definitive endoderm, 2) anterior foregut endoderm, 3) lung bud organoid, and 4) branching lung organoids. STEMdiff™ Branching Lung Organoid Kit has been optimized for differentiation of cells maintained in mTeSR™1 (Catalog #85850). Resulting organoids will develop proximal and distal-like branching airway epithelial structures expressing EPCAM, NKX2.1, SOX2, SOX9, MUC1, and P63. When maintained beyond 28 days in a Matrigel® sandwich, there is an increase in expression levels of markers for more mature lung cells, such as SFTPC, SFTPB, and ABCA3. For extended periods of organoid culture (> 28 days in the Matrigel® sandwich), the components required for branching lung organoid maturation are available in STEMdiff™ Branching Lung Organoid Maturation Kit (Catalog #100-0528).

Subtype
Specialized Media
 

Cell Type
Epithelial Cells, PSC-Derived, Mesenchymal Cells, PSC-Derived, Pluripotent Stem Cells
 

Species
Human
 

Application
Differentiation, Genome Editing, Organoid Culture
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Infectious Diseases, Respiratory Research, Transplantation Research
 

Formulation Category
Serum-Free

Product Advantages

- Physiologically relevant model system that recapitulates in vivo airway branching morphogenesis and proximo-distal specification

- Robust medium supports efficient differentiation of human ES and iPS cell lines to branching lung organoids

- Convenient format with cryopreservable intermediate stage for experimental flexibility

- Defined and serum-free medium system optimized to work with mTeSR™1 or mTeSR™ Plus

What's Included

- STEMdiff™ Endoderm Basal Medium (Lung), 100 mL

- STEMdiff™ Definitive Endoderm Supplement MR (100X), 0.35 mL

- STEMdiff™ Definitive Endoderm Supplement CJ (100X), 1.1 mL

- STEMdiff™ Lung Basal Medium, 180 mL

- STEMdiff™ Lung Supplement (10X), 20 mL

- STEMdiff™ Branching Lung Organoid Supplement A (100X), 0.25 mL

- STEMdiff™ Branching Lung Organoid Supplement B (100X), 1.75 mL

Catalog #: 100-0195

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.