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STEMdiff™ Branching Lung Organoid Maturation Kit

Serum-free medium for maturation of hPSC-derived branching lung organoids

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Overview

Mature and maintain human branching lung organoids in long-term (>28 days) culture using STEMdiff™ Branching Lung Organoid Maturation Kit.

Serum-free STEMdiff™ Branching Lung Organoid Maturation Kit may be used to reproducibly mature human embryonic stem (ES) and induced pluripotent stem (iPS) cell-derived lung bud organoids generated using STEMdiff™ Branching Lung Organoid Kit. Resulting organoids will develop proximal and distal-like branching airway epithelial structures expressing EPCAM, NKX2.1, SOX2, SOX9, MUC1, and P63. When maintained beyond 28 days in a Matrigel® sandwich, there is an increase in expression levels of markers for more mature lung cells, such as SFTPC, SFTPB, and ABCA3.

 

Subtype
Specialized Media
 

Cell Type
Epithelial Cells, PSC-Derived, Mesenchymal Cells, PSC-Derived, Pluripotent Stem Cells
 

Species
Human
 

Application
Differentiation, Genome Editing, Organoid Culture
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Infectious Diseases, Respiratory Research, Transplantation Research
 

Formulation Category
Serum-Free

 

What's Included

- STEMdiff™ Lung Basal Medium, 180 mL

- STEMdiff™ Lung Supplement (10X), 20 mL

- STEMdiff™ Branching Lung Organoid Supplement B (100X), 1.75 mL

Catalog #: 100-0528

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