Overview

Generate self-organized, pluripotent stem cell (PSC)-derived neural organoids with a cellular composition and structural organization representative of the developing human brain.

These defined, serum-free cell culture media and the simple, four-stage protocol are based on the formulation published by Lancaster et al. (Lancaster MA et al. Nature, 2013 and Lancaster MA et al. Science, 2014) to more reliably generate cerebral organoids. Beginning with an embryoid body (EB) formation step followed by expansion of neuroepithelia, organoids generated using STEMdiff™ Cerebral Organoid Kit feature cortical-like regions, including the ventricular zone (PAX6+/SOX2+/Ki-67+), outer subventricular zone (Ki-67+/p-Vimentin+), intermediate zone (TBR2+), and cortical plate (CTIP2+/MAP2+/TBR1+), which layer in similar orientations as those observed in vivo.

For extended culture periods (> 40 days), the components required for maturation can be purchased as STEMdiff™ Cerebral Organoid Maturation Kit.

Subtype
Specialized Media
 

Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture, Characterization, Differentiation, Functional Assay, Immunofluorescence, Organoid Culture, Phenotyping, Spheroid Culture
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Stem Cell Biology
 

Formulation Category
Serum-Free

What's Included

- STEMdiff™ Cerebral Organoid Basal Medium 1,100 mL

- STEMdiff™ Cerebral Organoid Basal Medium 2, 250 mL

- STEMdiff™ Cerebral Organoid Supplement A, 10 mL

- STEMdiff™ Cerebral Organoid Supplement B, 0.5 mL

- STEMdiff™ Cerebral Organoid Supplement C, 0.25 mL

- STEMdiff™ Cerebral Organoid Supplement D, 0.5 mL

- STEMdiff™ Cerebral Organoid Supplement E, 4.5 mL

Catalog #: 08570

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.