Overview

Take an in vitro approach to human neural biomarker discovery and central nervous system (CNS) permeability with human pluripotent stem cell (hPSC)-derived organoids patterned to the choroid plexus, the specialized brain epithelium that forms the blood-cerebrospinal fluid barrier. With a three-dimensional tissue structure and functional barrier epithelium, you’ll achieve unprecedented access and control in studying CNS permeability for drug distribution studies, as well as for production and extraction of cerebrospinal fluid (CSF).

Using STEMdiff™ Choroid Plexus Differentiation Kit’s simple five-stage protocol, you can efficiently and reproducibly generate choroid plexus organoids for a variety of downstream applications. The kit is based on the formulation published by Pellegrini et al. (Science, 2020), which was awarded a 3Rs Prize for its breakthroughs in animal reduction, replacement, and refinement. Choroid plexus organoid formation is initiated through an intermediate embryoid body (EB) formation step followed by expansion of neuroepithelia and patterning to choroid plexus-like epithelium. After a maturation period, organoids generated using this kit feature cystic structures filled with a fluid resembling CSF and surrounded by an epithelial layer expressing ependymal choroid plexus-specific markers (TTR, CLIC6, AQP1).

For extended periods of organoid culture (> 40 days), the components required for organoid maturation can be purchased as STEMdiff™ Choroid Plexus Organoid Maturation Kit (Catalog #100-0825).

Cell Type
Epithelial Cells, PSC-Derived, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture, Differentiation, Functional Assay, Organoid Culture, Phenotyping, Toxicity Assay
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Extracellular Vesicle Research, Neuroscience, Organoids
 

Formulation Category
Serum-Free

Product Advantages

- Adopt a relevant human CNS barrier model for screening and modeling applications with the precise control of an in vitro system

- Eliminate the need for complex co-cultures or Transwell® systems in your screening pipeline

- Extract cerebrospinal-like fluid to identify human CNS-specific biomarkers in a 100% blood-free system, without requiring a lumbar puncture

- Obtain 50X more cerebrospinal-like fluid from a single kit than obtained from a single mouse, and eliminate the donor-to-donor variability of pooled clinical samples

What's Included

- STEMdiff™ Neural Organoid Basal Medium 4, 250 mL

- STEMdiff™ Neural Organoid Supplement I, 4.5 mL

Catalog #: 100-0825

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.