Overview

STEMdiff™ Definitive Endoderm Kit is a complete, serum- and animal component-free medium and supplement kit that supports highly efficient differentiation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells to definitive endoderm cells. Cells differentiated using STEMdiff™ Definitive Endoderm Kit express high levels of endoderm markers, including CD184 (CXCR4), SOX17, FOXA2, and c-KIT, and lack expression of ectoderm, mesoderm, and pluripotency markers. The definitive endoderm cells produced using this kit are multipotent and capable of further differentiation towards cells of the pancreatic, intestinal, pulmonary, and hepatic lineages, thus providing a robust tool for developmental studies, disease modeling, and drug discovery.​

This kit is optimized for differentiation of cells maintained in mTeSR™1. For differentiation of cells maintained in TeSR™-E8™, please see the STEMDiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized).

Subtype
Specialized Media
 

Cell Type
Airway Cells, Endoderm, PSC-Derived, Hepatic Cells, Intestinal Cells, Pancreatic Cells, Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture, Differentiation
 

Brand
STEMdiff
 

Area of Interest
Cancer, Epithelial Cell Biology, Stem Cell Biology
 

Formulation Category
Animal Component-Free, Serum-Free

Product Advantages

- Defined, serum-free, animal component-free medium for the differentiation of human ES and iPS cells to definitive endoderm in a complete, ready-to-use format

- Efficient and reproducible differentiation of multiple ES cell and iPS cell lines

- Generates definitive endoderm cells capable of further differentiation to pancreatic, hepatic, intestinal and pulmonary cell lineages

What's Included

- STEMdiff™ Endoderm Basal Medium, 100 mL

- STEMdiff™ Definitive Endoderm Supplement MR (100X), 0.35 mL

- STEMdiff™ Definitive Endoderm Supplement CJ (100X), 1.1 mL

Catalog #: 05110

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.