Overview

Efficiently expand human pluripotent stem cell (hPSC)-derived endothelial cells using xeno-free STEMdiff™ Endothelial Expansion Culture Kit (Catalog #100-1218). This kit includes xeno-free STEMdiff™ Endothelial Expansion Basal Medium and 5X Supplement, and Animal Component-Free Cell Attachment Substrate, and is is optimized for the expansion of hPSC-derived endothelial cells and for the maintenance of endothelial marker expression. After 7 days of mesoderm and endothelial induction, the resulting cells can be expanded for 5 - 6 passages to generate large numbers of highly pure, mature endothelial cells that typically reach ≥ 99% CD144+CD31+ by passage 2 and maintain this phenotype until passage 5.

This kit is compatible with hPSCs maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), or TeSR™-E8™ (Catalog #05990) on Corning® Matrigel®. The components of STEMdiff™ Endothelial Expansion Culture Kit are included in STEMdiff™ Endothelial Differentiation Kit (Catalog #08005). For passaging, Animal Component-Free Cell Dissociation Kit (Catalog #05426) is required.

NOTE: Heparin Solution (Catalog #07980) is required for preparation of complete STEMdiff™ Endothelial Expansion Medium.

CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.

This product may be used for research purposes only. 

Cell Type
Endothelial Cells
 

Application
Cell Culture, Expansion
 

Brand
STEMdiff
 

Area of Interest
Angiogenic Cell Research, Disease Modeling, Endothelial Cell Biology

What's Included

- STEMdiff™ Endothelial Expansion Culture Kit (Catalog #100-1218)

    - STEMdiff™ Endothelial Expansion Basal Medium, 120 mL

    - STEMdiff™ Endothelial Expansion 5X Supplement, 30 mL

    - Animal Component-Free Cell Attachment Substrate, 1 mL

Catalog #: 100-1218

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.