Overview

STEMdiff™ Erythroid Kit is designed for the serum-free and feeder-free differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells to erythroid progenitor cells (erythroblasts) expressing Glycophorin A and CD71. This simple 2D differentiation protocol is performed in two stages. During the first 10- to 12-day stage, STEMdiff™ Hematopoietic Supplement A and then Supplement E1 are added to basal medium to induce cells toward erythroid-biased hematopoietic progenitor cells. At the end of this stage, hematopoietic progenitor cells are easily harvested from the culture supernatant. During the second 14-day stage, cells are further differentiated to erythroid progenitor cells using Supplement E2 and StemSpan™ SFEM II (Day 10 - 24). The cells typically expand 241-fold +/- 190-fold (95% CI) during the 14-day erythroid differentiation stage and the Day 24 cell population typically contains 74% +/- 5% (95 CI) CD71+GlyA+ erythroblasts. Cells generated using STEMdiff™ Erythroid Kit can be further matured into normoblasts and reticulocytes when moved to appropriate culture conditions for maturation.

STEMdiff™ Erythroid Kit has been optimized for differentiation of cells maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), and TeSR™-E8™ (Catalog #05990).

Subtype
Specialized Media
 

Cell Type
Erythroid Cells, Hematopoietic Stem and Progenitor Cells
 

Species
Human
 

Application
Cell Culture, Differentiation, Expansion
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology, Transplantation Research
 

Formulation Category
Serum-Free

Product Advantages

- Serum-free and feeder-free formulation

- Robust generation of erythroblasts across multiple ES and iPS cell lines

- Human iPSC/ESC-derived erythroblasts can be matured further

What's Included

- STEMdiff™ Hematopoietic Basal Medium, 120 mL

- STEMdiff™ Hematopoietic Supplement A, 225 μL

- STEMdiff™ Erythroid Supplement E1 (10X), 10 mL

- STEMdiff™ Erythroid Supplement E2 (10X), 20 mL

- StemSpan™ SFEM II, 100 mL

Catalog #: 100-0074

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.