Overview

Generate functional hematopoietic progenitor cells from human embryonic stem (ES) and induced pluripotent stem (iPS) cells with STEMdiff™ Hematopoietic Kit. Using a simple and reproducible protocol in serum- and feeder-free conditions, you can produce hematopoietic progenitor cells expressing CD34, CD45, and CD43.

The robust, two-stage protocol initially induces cells toward the mesoderm before further differentiating them into hematopoietic progenitor cells. After 12 days, a population of hematopoietic cells containing 25 - 65% (average 43%) CD34+CD45+ progenitor cells can be harvested, including progenitor cells that have the capacity to form functional hematopoietic colonies in the colony-forming unit (CFU) assay.

STEMdiff™ Hematopoietic Kit has been optimized for differentiation of human pluripotent cells maintained in:
-  mTeSR™1 (Catalog #85850)
- mTeSR™ Plus (Catalog #100-0276)
- TeSR™-E8™ (Catalog #05990)

Subtype
Specialized Media
 

Cell Type
Hematopoietic Stem and Progenitor Cells, Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture, Differentiation
 

Brand
STEMdiff
 

Area of Interest
Stem Cell Biology
 

Formulation Category
Serum-Free

What's Included

- STEMdiff™ Hematopoietic Basal Medium, 120 mL

- STEMdiff™ Hematopoietic Supplement A (200X), 225 µL

- STEMdiff™ Hematopoietic Supplement B (200X), 375 µL

Catalog #: 05310

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.