Overview

STEMdiff™ Intestinal Organoid Kit (Catalog #05140) is a serum-free cell culture medium system that enables robust and efficient generation of human pluripotent stem cell (hPSC)-derived intestinal organoids in a simple, three-stage protocol. Using this kit, hPSCs are directed through induction of definitive endoderm, mid-/hindgut spheroids, and generation of intestinal organoids that can be maintained long-term through passaging or cryopreserved. The organoids exhibit a cellular composition and organization that models the developing intestinal epithelium and associated mesenchyme, making them a convenient model system with direct relevance to the developing intestine. STEMdiff™ Intestinal Organoid Kit is based on the formulation published by Spence et al. (Nature 2011) and has been optimized to increase efficiency and reproducibility of organoid formation and expansion across hPSC lines. Human intestinal organoids can be used as a model system for studying intestinal development and cell biology, intestinal inflammation, intestinal regeneration, microbial interaction, disease modeling, drug discovery, and compound screening. The kit is optimized for differentiation of cells maintained in mTeSR™1.

For extended culture and passaging, the kit components required for organoid maintenance can be purchased as STEMdiff™ Intestinal Organoid Growth Medium (Catalog #05145).

Cell Type
Endoderm, PSC-Derived, Intestinal Cells
 

Species
Human
 

Application
Cell Culture, Differentiation, Organoid Culture
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Relevant model system for the developing small intestinal epithelium and associated mesenchyme

- Robust medium supports efficient differentiation of human ES and iPS cell lines to intestinal organoids

- Convenient model system that can be maintained long-term through passaging or cryopreserved for experimental flexibility

- Serum-free medium system optimized for low experimental variability

What's Included

- STEMdiff™ Endoderm Basal Medium,100 mL

- STEMdiff™ Definitive Endoderm Supplement CJ, 1.1 mL

- STEMdiff™ Gastrointestinal Supplement PK, 0.64 mL

- STEMdiff™ Gastrointestinal Supplement UB, 0.64 mL

- STEMdiff™ Intestinal Organoid Basal Medium, 100 mL

- STEMdiff™ Intestinal Organoid Supplement, 2 mL

Catalog #: 05140

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.