Overview

STEMdiff™ Lung Progenitor Kit is a serum-free culture medium system for efficient and reproducible generation of lung progenitor cells from human embryonic stem (ES) and induced pluripotent stem (iPS) cells through three stages of differentiation: 1) definitive endoderm, 2) anterior foregut endoderm, and 3) lung progenitor cells. Differentiated cells will express NKX2.1, a key marker of lung progenitor cells. The resulting cells can be further matured towards proximal or distal airway cells using published protocols for the study of lung diseases and lung development.

Subtype
Specialized Media
 

Cell Type
Airway Cells, Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture, Differentiation, Organoid Culture
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Respiratory Research, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Differentiate hPSCs to lung progenitor cells with a robust medium, optimized to work with mTeSR™1

- Obtain lung progenitor cells capable of downstream differentiation to proximal and distal airway cells

- Maximize experimental reproducibility with an optimized, serum-free formulation

- Convenient format and easy-to-use protocol

What's Included

- STEMdiff™ Endoderm Basal Medium, 100 mL

- STEMdiff™ Definitive Endoderm Supplement MR (100X), 0.35 mL

- STEMdiff™ Definitive Endoderm Supplement CJ (100X), 1.1 mL

- STEMdiff™ Lung Basal Medium, 180 mL

- STEMdiff™ Lung Supplement (10X), 20 mL

- STEMdiff™ Lung Progenitor Supplement 1 (100X), 0.55 mL

- STEMdiff™ Lung Progenitor Supplement 2 (100X), 1.45 mL

Catalog #: 100-0230

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.