Overview

STEMdiff™ Megakaryocyte Kit is designed for the serum-free and feeder-free differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells to polyploid megakaryocytes capable of shedding platelets and expressing CD41a and CD42b. This simple two-dimensional 17-day protocol includes two stages.

During the first 12 days, STEMdiff™ Hematopoietic Supplement A and then Supplement MK1 are added to basal medium to induce cells toward megakaryocyte-biased hematopoietic progenitor cells. At the end of this stage, hematopoietic progenitor cells are easily harvested from the culture supernatant, and are further differentiated to mature megakaryocytes using Supplement MK2 and StemSpan™ SFEM II for an additional 5 days.

At the end of the protocol (Day 17), the cells are typically expanded 405-fold +/- 54-fold (95% CI), and on average 71% +/- 3.3% (95 CI) of cells co-express CD41a and CD42b.

STEMdiff™ Megakaryocyte Kit has been optimized for differentiation of ES/iPS cells maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), or TeSR™-AOF (Catalog #100-0401).

Subtype
Specialized Media
 

Cell Type
Hematopoietic Cells, PSC-Derived, Hematopoietic Stem and Progenitor Cells, Megakaryocytes
 

Application
Differentiation
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology, Cell Therapy Development
 

Formulation Category
Serum-Free

Product Advantages

- Serum-free and feeder-free formulation

- Generates high yields of megakaryocytes per input hPSC

- iPSC/ESC-derived megakaryocytes show high ploidy and platelet-shedding ability

- Amenable to large-scale culture (feeder-free)

What's Included

- STEMdiff™ Hematopoietic Basal Medium, 120 mL

- STEMdiff™ Hematopoietic Supplement A (200X), 225 μL

- STEMdiff™ Megakaryocyte Supplement MK 1 (10X), 10 mL

- STEMdiff™ Megakaryocyte Supplement MK 2 (10X), 10 mL

- StemSpan™ SFEM II, 100 mL (Catalog #09605)

Catalog #: 100-0900

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.