Overview

The STEMdiff™ Microglia Culture System comprises STEMdiff™ Microglia Differentiation Kit and STEMdiff™ Microglia Maturation Kit. Together, these kits are used to differentiate and mature microglia derived from human pluripotent stem cells (hPSCs) using STEMdiff™ Hematopoietic Kit (Catalog #05310).

Based on the protocol from the laboratory of Mathew Blurton-Jones (Abud et al., 2017), the resulting cells are a highly pure population of microglia (> 80% CD45/CD11b-positive, > 50% TREM2-positive microglia; < 20% morphologically distinct monocytes or macrophages).

Cells derived using these products are versatile tools for modeling neuroinflammation, studying human neurological development and disease, co-culture applications, and toxicity testing.

Subtype
Specialized Media
 

Cell Type
Hematopoietic Cells, PSC-Derived, Microglia, Neural Cells, PSC-Derived
 

Species
Human
 

Application
Cell Culture, Differentiation
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Immunology, Neuroscience
 

Formulation Category
Serum-Free

Product Advantages

- Greater than 90% of source HPCs are differentiated into microglia.

- Upstream compatibility with HPCs generated using the STEMdiff™ Hematopoietic Kit and co-culture with STEMdiff™ neural organoids.

- Allows differentiation to microglia with few contaminating macrophages or monocytes.

- Microglia generated with the kit demonstrate phagocytosis and activation.

- Required materials are provided in a simple, easy-to-use format.

What's Included

- STEMdiff™ Microglia Maturation Kit (Catalog #100-0020)

    - STEMdiff™ Microglia Basal Medium, 90 mL

    - STEMdiff™ Microglia Supplement 1, 10 mL

    - STEMdiff™ Microglia Supplement 2, 400 uL

    - STEMdiff™ Microglia Supplement 3, 400 uL

Catalog #: 100-0020

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.