Overview

STEMdiff™ Midbrain Neuron Differentiation Kit (Catalog #100-0038) is used to generate midbrain neuronal precursors from neural progenitor cells (NPCs) derived from human pluripotent stem cells (hPSCs) using STEMdiff™ SMADi Neural Induction Kit (Catalog #08581). The midbrain neuronal precursors are further matured into midbrain neurons using STEMdiff™ Midbrain Maturation Kit (Catalog #100-0041). These media will produce a population of midbrain neurons (≥ 15% TH-positive dopaminergic neurons; ≥ 90% class III βtubulin-positive neurons; < 10% GFAP-positive astrocytes). Cells derived using these products are versatile tools for modeling human neurological development and disease, drug screening, toxicity testing, and cell therapy validation.

Subtype
Specialized Media
 

Cell Type
Dopaminergic Neurons, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells
 

Species
Human
 

Application
Cell Culture, Differentiation
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience
 

Formulation Category
Serum-Free

Product Advantages

- Defined and serum-free

- Supports highly efficient generation of midbrain-like functional neurons from hPSC-derived neuronal precursors

- Produces a population of TH-positive neurons (> 15%) that can be maintained long term in culture

- Optimized for differentiation of neuronal progenitor cells generated using STEMdiff™ SMADi Neural Induction Kit

- Enables reproducible generation of midbrain-type neuronal precursors derived from multiple human ES and iPS cell lines

What's Included

- STEMdiff™ Midbrain Neuron Differentiation Basal Medium, 80 mL

- STEMdiff™ Midbrain Neuron Differentiation Supplement, 20 mL

Catalog #: 100-0038

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.