Overview

Robustly generate three-dimensional, patterned brain organoid cultures from human pluripotent stem cells without matrix embedding by using STEMdiff™ Midbrain Organoid Differentiation Kit. When paired with AggreWell™800™, this serum-free cell culture media can prevent organoid fusion and support the generation of over 500 organoids per kit for higher-powered statistical replicates and more detailed longitudinal study.

Adapted from protocols by Dr. Sergiu Paşca, the brain-region-specific organoids generated with this kit are three-dimensional in vitro models with a cellular composition and structural organization representative of the developing human midbrain. They are consistently sized with reproducible morphology between various cell lines, and provide a reliable model for the study of dopaminergic neurons within a functional 3D system.

Midbrain organoids can be combined with striatal organoids generated using the STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit with additional patterning factors to generate midbrain-striatal AssemBloid™ cultures. Organoids can be maintained with STEMdiff™ Neural Organoid Maintenance Kit to support long-term culture survival (> 50 days) for predictive assays, high-throughput phenotypic screening, and neurotoxicity assays.

Subtype
Specialized Media
 

Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells
 

Application
Cell Culture, Characterization, Differentiation, Functional Assay, Immunofluorescence, Organoid Culture, Phenotyping, Spheroid Culture
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Organoids
 

Formulation Category
Serum-Free

Product Advantages

- Eliminate organoid embedding steps with a matrix-free system

- Obtain consistently sized organoids with reproducible morphology between cell lines

- Generate over 500 organoids per kit using AggreWell™800

- Achieve long-term culture survival for predictive assays, high-throughput phenotypic screening, and neurotoxicity assays

- Combine modular region-patterned organoids to generate advanced AssemBloids™

What's Included

- STEMdiff™ Neural Organoid Basal Medium 1, 20 mL

- STEMdiff™ Neural Organoid Basal Medium 2, 500 mL

- STEMdiff™ Neural Organoid Supplement A, 10 mL

- STEMdiff™ Neural Organoid Supplement C, 0.25 mL

- STEMdiff™ Neural Organoid Supplement K, 0.5 mL

- STEMdiff™ Neural Organoid Supplement L, 0.5 mL

Catalog #: 100-1096

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.