Overview

Differentiate human pluripotent stem cells (hPSCs) to monocytes expressing CD14 with the feeder-free and serum-free STEMdiff™ Monocyte Kit.

The simple protocol is performed in 2D adherent cultures. During the first 3 days, Medium A induces cells toward mesoderm. For the subsequent 4 days, mesodermal cells are further differentiated toward the hematopoietic lineage using Medium B. At Day 7, the medium is changed to Monocyte Differentiation Medium, which facilitates the differentiation to monocytes. CD14⁺ monocytes can be harvested directly from the culture supernatant starting as early as Day 14 and can be repeatedly harvested during the rest of the culture period. Peak CD14⁺ frequency is typically 60 - 80%.

The hPSC-derived monocytes can be further differentiated to dendritic cells or macrophages using the ImmunoCult™ Dendritic Cell Culture Kit or the ImmunoCult™-SF Macrophage Medium, respectively.

For your convenience, components required for the preparation of the Monocyte Differentiation Medium, StemSpan™ SFEM II and STEMdiff™ Monocyte Differentiation Supplement (100X), are also available for purchase individually.

Subtype
Specialized Media
 

Cell Type
Dendritic Cells, Macrophages, Monocytes, Myeloid Cells, Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture, Differentiation, Expansion
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Immunology, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Serum-free and feeder-free formulation

- Simple monolayer protocol produces monocytes in suspension for easy harvest

- Generation of CD14⁺ monocytes in 14 - 23 days

- Robust generation of monocytes across multiple hPSC cell lines

What's Included

- STEMdiff™ Hematopoietic Basal Medium, 120 mL

- STEMdiff™ Hematopoietic Supplement A (200X), 225 μL

- STEMdiff™ Hematopoietic Supplement B (200X), 225 μL

- STEMdiff™ Monocyte Differentiation Supplement (100X), 3 x 1 mL (Catalog #05324)

- StemSpan™ SFEM II, 3 x 100 mL

Catalog #: 05320

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.