Overview

With the STEMdiff™ Motor Neuron Differentiation Kit, based on the established protocol by Maury et al. (Nature Biotechnology, 2015), you can produce pure populations of spinal motor neurons with an average of >80% Class III β-tubulin positive, >50% motor neuron marker ISL1 positive, >60% motor neuron marker HB9 positive. These motor neurons also exhibit high expression of HOXA5. Use the resulting motor neuron cultures in a variety of applications, such as modeling human neurological disease or toxicity testing. These highly versatile cultures are suitable for use in neuromuscular co-culture with myotubes generated using the MyoCult™ Differentiation Kit (Catalog #05965), in co-culture with microglia generated using the STEMdiff™ Microglia Differentiation Kit (Catalog #100-0019), or with AggreWell™400 (Catalog #34460) for higher yield production.

For further maturation of motor neurons generated with this kit, pair with the STEMdiff™ Motor Neuron Maturation Kit to produce >40% ChAT cells in an additional 14 days.

Subtype
Specialized Media
 

Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons
 

Species
Human
 

Application
Cell Culture, Differentiation
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience
 

Formulation Category
Serum-Free

Product Advantages

- Differentiate motor neurons from human induced pluripotent stem cell lines (iPSCs) in only 14 days
- Streamline motor neuron culture with a simple, scalable workflow
- Produce physiologically relevant results with integrated BrainPhys™ Neuronal Medium, supporting neuronal activity and maturation
- Model the complexities of cell-cell interactions by pairing with compatible differentiation kits for co-culture applications

What's Included

- STEMdiff™ Motor Neuron Differentiation Basal Medium, 100 mL

- STEMdiff™ Motor Neuron Differentiation Supplement A, 120 μL

- STEMdiff™ Motor Neuron Differentiation Supplement B, 200 μL

- STEMdiff™ Motor Neuron Differentiation Supplement C, 120 μL

Catalog #: 100-0871

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.