Overview

Culture patterned brain organoids for extended periods (> 50 days) without the use of embedding matrices. With the essential components to prevent organoid fusion and retain a high yield of reproducible organoids, STEMdiff™ Neural Organoid Maintenance Kit is a serum-free cell culture medium designed for the maintenance and maturation of neural organoids adapted from protocols by Sergiu Paşca (F Birey et al. Nature, 2017). When used following STEMdiff™ Dorsal or Ventral Forebrain Organoid Differentiation Kits (Catalog #08620 and #08630), STEMdiff™ Neural Organoid Maintenance Kit allows for long-term organoid experiments for neurodegeneration research, biomarker discovery, and toxicity assays. The resulting brain-region-specific organoids have a cellular composition and structural organization that is representative of the developing human forebrain. Organoids generated with these kits can also be co-cultured as assembloids to study brain region interactions (F Birey et al. Nature, 2017).

Subtype
Specialized Media
 

Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells
 

Species
Human
 

Application
Characterization, Differentiation, Functional Assay, Immunofluorescence, Maintenance, Organoid Culture, Phenotyping, Spheroid Culture
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Organoids
 

Formulation Category
Serum-Free

Product Advantages

- Reduce handling and media waste with fusion-free growth media

- Obtain greater analytic sensitivity for disease phenotypes with reproducible morphology between lines and individual organoids

- Eliminate matrix embedding steps with the matrix-free formulation and protocol

- Easily process and section consistently sized organoids

- Achieve long-term culture survival and reduced caspase-3 expression for neurotoxicity and neurodegenerative models

- Combine modular region-patterned organoids to generate advanced AssemBloids™ for disease modeling and regenerative applications

What's Included

 STEMdiff™ Neural Organoid Basal Medium 2, 500 mL

- STEMdiff™ Neural Organoid Supplement A, 10 mL

Catalog #: 100-0120

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.