Overview

STEMdiff™ Pancreatic Progenitor Kit is a serum-free medium that supports efficient and reproducible generation of pancreatic progenitor cells from human pluripotent stem cells (hPSCs). Cells are differentiated through four stages: 1) definitive endoderm, 2) primitive gut tube, 3) posterior foregut endoderm, and 4) pancreatic progenitor cells. The differentiated cells express key markers of pancreatic progenitor cells, including PDX-1, NKX6.1 and SOX9 and up-regulation of insulin and glucagon. The resulting pancreatic progenitor cells can be further differentiated to insulin-producing beta cells or other endocrine and exocrine pancreatic cell fates.

STEMdiff™ Pancreatic Progenitor Kit has been optimized for differentiation of cells maintained in mTeSR™1 (Catalog #85850) or mTeSR™ Plus (Catalog #100-0276).

Subtype
Specialized Media
 

Cell Type
Pancreatic Cells, Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture, Differentiation
 

Brand
STEMdiff
 

Area of Interest
Cancer, Disease Modeling, Epithelial Cell Biology, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Generates functional pancreatic progenitor cells for transplantation into animal models or for further maturation into insulin-producing beta cells

- Efficient and reproducible differentiation of multiple hPSC lines to pancreatic progenitor cells expressing PDX-1, NKX6.1, and SOX9

- Serum-free medium

- Compatible with multiple human ES and iPS cell lines maintained in mTeSR™1 or mTeSR™ Plus.

What's Included

- STEMdiff™ Endoderm Basal Medium, 100 mL

- STEMdiff™ Pancreatic Stage 2 - 4 Basal Medium, 265 mL

- STEMdiff™ Definitive Endoderm Supplement MR (100X), 350 µL

- STEMdiff™ Definitive Endoderm Supplement CJ (100X), 1.1 mL

- STEMdiff™ Pancreatic Supplement 2A, 240 µL

- STEMdiff™ Pancreatic Supplement 2B, 720 µL

- STEMdiff™ Pancreatic Supplement 3, 720 µL

- STEMdiff™ Pancreatic Supplement 4, 1.2 mL

Catalog #: 05120

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.