Overview

Generate functional, human-specific sensory neurons in a dish with this easy-to-use culture system. After obtaining pluripotent stem cell (PSC)-derived neural crest cells (NCCs) with STEMdiff™ Neural Crest Differentiation Kit, use the serum-free STEMdiff™ Sensory Neuron Differentiation Kit to generate sensory neuron precursors. STEMdiff™ Sensory Neuron Maturation Kit can then be used to maintain and mature the sensory neuron precursors. The resulting cells are BRN3A-positive and more than 70% TUJ1-positive. With the physiological glucose and osmolarity conditions provided by BrainPhys™ as the basal medium, the neurons exhibit activity in response to sensory ligands and changes in temperature. The generated population of functional human sensory neurons can be used for drug discovery and pain research applications.

Subtype
Specialized Media
 

Cell Type
Neural Cells, PSC-Derived, Neurons
 

Species
Human
 

Application
Cell Culture, Differentiation
 

Brand
BrainPhys, STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience
 

Formulation Category
Serum-Free

Product Advantages

- Reduce animal usage by eliminating costly dorsal root ganglia explant workflows

- Differentiate sensory neurons from multiple human induced pluripotent stem (iPS) cell lines using an NCC intermediate and the STEMdiff™ Neural Crest Differentiation Kit

- Streamline PSC-derived sensory neuron generation with a simple, easy-to-use media format

- Obtain physiologically relevant results with integrated BrainPhys™ media supporting neuronal activity and maturation

What's Included

- STEMdiff™ Sensory Neuron Differentiation Basal Medium, 100 mL

- STEMdiff™ Sensory Neuron Differentiation Supplement, 1 mL

Catalog #: 100-0341

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.