Overview

Generate functional, human-specific sensory neurons in a dish with this easy-to-use culture system. STEMdiff™ Sensory Neuron Maturation Kit is the final step in a workflow beginning from human pluripotent stem cells (hPSCs) and leading to a population of sensory neurons that can be used for drug discovery and pain research applications. After obtaining PSC-derived neural crest cells (NCCs) with STEMdiff™ Neural Crest Differentiation Kit, use the serum-free STEMdiff™ Sensory Neuron Differentiation Kit to generate sensory neuron precursors. STEMdiff™ Sensory Neuron Maturation Kit can then be used to maintain and mature the sensory neuron precursors. The resulting cells are BRN3A-positive and more than 70% TUJ1-positive. With the physiological glucose and osmolarity conditions provided by BrainPhys™ as the basal medium, the neurons exhibit activity in response to sensory ligands and changes in temperature.

Subtype
Specialized Media
 

Cell Type
Neural Cells, PSC-Derived, Neurons
 

Species
Human
 

Application
Cell Culture, Characterization, Differentiation, Functional Assay, Immunocytochemistry, Phenotyping
 

Brand
BrainPhys, STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience
 

Formulation Category
Serum-Free

Product Advantages

- Reduce animal usage by eliminating costly dorsal root ganglia explant workflows

- Mature and maintain sensory neurons seamlessly following STEMdiff™ Sensory Neuron Differentiation Kit

- Streamline PSC-derived sensory neuron generation with a simple, easy-to-use media format

- Obtain physiologically relevant results with integrated BrainPhys™ media supporting neuronal activity and maturation

What's Included

- BrainPhys™ Neuronal Medium, 100 mL (Catalog #05797)

- STEMdiff™ Sensory Neuron Maturation Supplement, 25 mL

Catalog #: 100-0684

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.