Overview

STEMdiff™ SMADi Neural Induction Kit consists of a defined, serum-free medium and supplement for the highly efficient neural induction of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. This kit combines STEMdiff™ Neural Induction Medium (Catalog #05835) with STEMdiff™ SMADi Neural Induction Supplement, which directs differentiation by blocking TGF-β/BMP-dependent SMAD signaling, resulting in efficient neural induction of even hard-to-differentiate cell lines. Neural progenitor cells (NPCs) can be generated using STEMdiff™ SMADi Neural Induction Kit with either an embryoid body (EB) protocol or monolayer culture protocol. The resulting cultures are enriched for central nervous system (CNS)-type NPCs, which express SOX1, Nestin, and PAX6. NPCs generated using this kit can be passaged as single cells and expanded in STEMdiff™ Neural Progenitor Medium (Catalog #05833). The NPCs can also be differentiated into neurons and glia.

Subtype
Specialized Media
 

Cell Type
Neural Cells, PSC-Derived, Pluripotent Stem Cells
 

Application
Differentiation
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Defined and serum-free

- Promotes efficient conversion of ES and iPS cells to CNS-type NPCs, and inhibits unwanted differentiation of non-CNS cell types

- Highly efficient neural induction of even hard-to-differentiate ES and iPS cell lines

- Improves efficiency of downstream differentiation into neurons and glia

- Compatible with both embryoid body and monolayer culture protocols for neural induction

- Enables reproducible differentiation of cell lines maintained in any TeSR™ family maintenance medium

- Convenient, user-friendly format and protocols

What's Included

- STEMdiff™ SMADi Neural Induction Kit (Catalog #08581)

    - STEMdiff™ Neural Induction Medium, 250 mL

    - STEMdiff™ SMADi Neural Induction Supplement, 0.5 mL

Catalog #: 08581 (1 Kit), 08582 (2 Kits)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.