Overview

Robustly generate three-dimensional, patterned spinal cord organoid cultures from human pluripotent stem cells without matrix embedding by using STEMdiff™ Spinal Cord Organoid Differentiation Kit. When paired with AggreWell™800, this serum-free cell culture media can prevent organoid fusion and support the generation of up to 300 organoids per kit for higher-powered statistical replicates and more detailed longitudinal study.

Adapted from protocols by Dr. Sergiu Paşca (Andersen J et al. Cell, 2020; Yoon SJ et al. Nature Methods, 2018), the spinal cord organoids generated with this kit are 3D in vitro models with a cellular composition and structural organization representative of the developing human cervical spinal cord. They are consistently sized with reproducible morphology between various cell lines and provide a reliable model for the study of motor neurons within a functional 3D system.

Organoids generated with this kit can also be co-cultured as AssemBloids™ to study brain region interactions (Andersen J et al. Cell, 2020). For extended periods of organoid culture (> 50 days), organoids can be maintained with STEMdiff™ Neural Organoid Maintenance Kit to support long-term culture survival for predictive assays, high-throughput phenotypic screening, and neurotoxicity assays.

Subtype
Specialized Media
 

Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells
 

Application
Cell Culture, Characterization, Differentiation, Functional Assay, Immunofluorescence, Organoid Culture, Phenotyping, Spheroid Culture
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Organoids
 

Formulation Category
Serum-Free

Product Advantages

- Eliminate organoid embedding steps with a matrix-free system

- Obtain consistently sized organoids with reproducible morphology between cell lines

- Generate up to 300 organoids per kit by using with AggreWell™800

- Achieve long-term culture survival for predictive assays, high-throughput phenotypic screening, and neurotoxicity assays

- Combine modular region-patterned organoids to generate advanced AssemBloids™

What's Included

- STEMdiff™ Neural Organoid Basal Medium 1

- STEMdiff™ Neural Organoid Basal Medium 2

- STEMdiff™ Neural Organoid Supplement A

- STEMdiff™ Neural Organoid Supplement C

- STEMdiff™ Neural Organoid Supplement M

- STEMdiff™ Neural Organoid Supplement N

- STEMdiff™ Neural Organoid Supplement O

- STEMdiff™ Neural Organoid Supplement P

Catalog #: 100-1524

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.