Overview

Differentiate human pluripotent stem cells (hPSCs) to T cells with the feeder-free and serum-free STEMdiff™ T Cell Kit.

The STEMdiff™ T Cell Kit protocol first uses animal component-free STEMdiff™ Hematopoietic - EB reagents for the generation of embryoid bodies (EBs) from hPSCs which are then further differentiated into CD34⁺ cells. The kit includes all the EB reagents necessary for this step:
• STEMdiff™ Hematopoietic - EB Basal Medium
• STEMdiff™ Hematopoietic - EB Supplement A
• STEMdiff™ Hematopoietic - EB Supplement B

The CD34⁺ cells are then further directed to differentiate to T cells using StemSpan™ reagents:
• StemSpan™ SFEM II
• StemSpan™ Lymphoid Progenitor Expansion Supplement (10X)
• StemSpan™ Lymphoid Differentiation Coating Material (100X)
• StemSpan™ T Cell Progenitor Maturation Supplement (10X)

For your convenience, all STEMdiff™ Hematopoietic - EB and StemSpan™ reagents included with the kit are also available individually.

Subtype
Specialized Media
 

Cell Type
Hematopoietic Cells, PSC-Derived, Pluripotent Stem Cells, T Cells, T Cells, CD8+
 

Species
Human
 

Application
Cell Culture, Differentiation, Expansion
 

Brand
STEMdiff
 

Area of Interest
Cancer, Disease Modeling, Immunology, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Serum-free and feeder-free formulation

- Uniform generation of embryoid bodies

- Robust generation of CD4+CD8+ double-positive T cells across multiple ES and iPS cell lines

What's Included

- STEMdiff™ Hematopoietic - EB Basal Medium, 120 mL (Catalog #100-0171)

- STEMdiff™ Hematopoietic - EB Supplement A, 265 μL (Catalog #100-0172)

- STEMdiff™ Hematopoietic - EB Supplement B, 7 mL (Catalog #100-0173)

- StemSpan™ SFEM II, 2 x 100 mL (Catalog #09605)

- StemSpan™ Lymphoid Progenitor Expansion Supplement (10X), 5 mL (Catalog #09915)

- StemSpan™ Lymphoid Differentiation Coating Material (100X), 2 x 0.25 mL (Catalog #09925)

- StemSpan™ T Cell Progenitor Maturation Supplement (10X), 12.5 mL (Catalog #09930)

Catalog #: 100-0194

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.