Overview

The STEMdiff™ Trilineage Differentiation Kit provides a simple culture assay to functionally validate the ability of new or established human embryonic stem (ES) and induced pluripotent stem (iPS) cell lines to differentiate to the three germ layers: ectoderm, mesoderm, and endoderm. This kit includes specialized, complete media and monolayer-based protocols to perform parallel in vitro directed differentiation experiments for each germ layer, clearly and reproducibly establishing trilineage differentiation potential within one week. STEMdiff™ Trilineage Differentiation Kit is intended to be an endpoint assay and is not optimized for the generation of cells for downstream differentiation or other applications. STEMdiff™ Trilineage Differentiation Kit has been optimized to assess cells maintained in mTeSR™1, mTeSR™ Plus, or TeSR™- AOF.

Subtype
Specialized Media
 

Cell Type
Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture, Characterization, Differentiation, Functional Assay, Phenotyping
 

Brand
STEMdiff
 

Area of Interest
Stem Cell Biology

Product Advantages

- Reproducible differentiation to all three germ layers across multiple pluripotent cell lines

- Easy-to-interpret assay results

- Complete, defined culture media

- Standardized, one-week protocol

What's Included

- STEMdiff™ Trilineage Ectoderm Medium, 175 mL

- STEMdiff™ Trilineage Mesoderm Medium, 100 mL

- STEMdiff™ Trilineage Endoderm Medium, 100 mL

Catalog #: 05230

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.