Overview

Robustly generate three-dimensional, patterned brain organoid cultures from human pluripotent stem cells without matrix embedding. STEMdiff™ Dorsal and Ventral Forebrain Organoid Differentiation Kits are serum-free cell culture media that work with AggreWell™-generated embryoid bodies to prevent organoid fusion and enable the scalable generation of over 500 highly reproducible organoids per kit. Adapted from protocols by Sergiu Paşca (F Birey et al. Nature, 2017), these brain-region-specific organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative of the developing human forebrain. STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit (Catalog #08620) generates tissue of the early developing dorsal pallium, while STEMdiff™ Ventral Forebrain Organoid Differentiation Kit (Catalog #08630) generates tissue of the early developing ventral subpallium. Organoids generated with these kits can also be co-cultured as assembloids to study brain region interactions (F Birey et al. Nature, 2017). For extended periods of organoid culture (> 50 days), the components required for organoid maintenance are available as STEMdiff™ Neural Organoid Maintenance Kit (Catalog #100-0120).

Subtype
Specialized Media
 

Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells
 

Species
Human
 

Application
Cell Culture, Characterization, Differentiation, Functional Assay, Immunofluorescence, Organoid Culture, Phenotyping, Spheroid Culture
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Organoids
 

Formulation Category
Serum-Free

Product Advantages

- Reduce handling and media waste with fusion-free growth media

- Obtain greater analytic sensitivity for disease phenotypes with reproducible morphology between lines and individual organoids

- Generate over 500 organoids per kit using AggreWell™800 for higher-powered statistical replicates and more detailed longitudinal study

- Eliminate matrix embedding steps with the matrix-free formulation and protocol

- Easily process and section consistently sized organoids

- Achieve long-term culture survival and reduced caspase-3 expression for neurotoxicity and neurodegenerative models

- Combine modular region-patterned organoids to generate advanced AssemBloids™ for disease modeling and regenerative applications

What's Included

- STEMdiff™ Neural Organoid Basal Medium 1, 20 mL

- STEMdiff™ Neural Organoid Basal Medium 2, 500 mL

- STEMdiff™ Neural Organoid Supplement A, 10 mL

- STEMdiff™ Neural Organoid Supplement B, 0.25 mL

- STEMdiff™ Neural Organoid Supplement C, 0.25 mL

- STEMdiff™ Neural Organoid Supplement D, 0.5 mL

Catalog #: 08630

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.