Overview

STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit (Catalog #05010) includes a medium for differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells (human pluripotent stem cells [hPSCs]) into ventricular cardiomyocytes (cardiac troponin T-positive [cTnT+]), as well as a medium for maintenance of hPSC-derived cardiomyocytes. This serum-free kit can be used to generate ventricular cardiomyocytes derived from a clump culture of hPSCs maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), TeSR™-AOF (Catalog #100-0401), or TeSR™-E8™ (Catalog #05990). Greater than 80% of these cells will be cTnT+. An average of 1 x 10^6 cells can be harvested from a single well of a 12-well plate.

STEMdiff™ Cardiomyocyte Maintenance Kit (Catalog #05020) comprises the maintenance basal medium and supplement; it can be used for long-term maintenance of hPSC-derived cardiomyocytes for one month or longer. These cardiomyocytes can be used in various downstream applications and analyses.

NOTE: This product was formerly named ‘STEMdiff™ Cardiomyocyte Differentiation Kit’; the product itself and manufacturing procedures have not changed, but the name has been updated to more accurately reflect the cell type generated.

Subtype
Specialized Media
 

Cell Type
Cardiomyocytes, PSC-Derived
 

Species
Human
 

Application
Cell Culture, Differentiation, Maintenance
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology
 

Formulation Category
Serum-Free

Product Advantages

- Supports the entire hPSC-derived cardiomyocyte workflow

- Simple monolayer protocol produces cardiomyocytes in 15 days

- One serum-free kit generates over 50 million cardiomyocytes (cTnT+)

- Robust performance with minimal variability across multiple hPSC lines

What's Included

- STEMdiff™ Cardiomyocyte Differentiation Basal Medium, 380 mL

- STEMdiff™ Ventricular Cardiomyocyte Differentiation Supplement A (10X), 10 mL

- STEMdiff™ Ventricular Cardiomyocyte Differentiation Supplement B (10X), 10 mL

- STEMdiff™ Ventricular Cardiomyocyte Differentiation Supplement C (10X), 20 mL

- STEMdiff™ Cardiomyocyte Maintenance Basal Medium, 490 mL

- STEMdiff™ Cardiomyocyte Maintenance Supplement (50X), 10 mL

Catalog #: 05010

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.