Overview

Rapidly and robustly generate hPSC-derived immature retinal pigment epithelium (RPE) in 14 days with STEMdiff™-ACF RPE Differentiation Kit. This animal component- and serum-free kit generates immature RPE (> 50% PMEL17+) from hPSCs in clump culture maintained in mTeSR™1 or mTeSR™ Plus.

Immature RPE generated using STEMdiff™-ACF RPE Differentiation Kit can be cryopreserved as an intermediate cell bank or matured further into functional RPE using STEMdiff™-XF RPE Maturation Medium. By using these products together, a highly pure population of RPE (Figure 2) can be generated from hPSCs in as few as 49 days without the need for manual selection or cell enrichment. Increase the yields and survivability of your RPE cell differentiation and maturation by adding the STEMdiff™-ACF RPE Plating Supplement to this workflow.

Cells derived using these products can be used for modeling human retinal development and disease, drug screening, cell and gene therapy validation, and advanced tissue model development.

Cell Type
Neural Cells, PSC-Derived, Pluripotent Stem Cells
 

Application
Cell Culture, Differentiation
 

Brand
STEMdiff
 

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Cell Therapy Development

Product Advantages

- Get consistent results with an animal component- and serum-free formulation

- Streamline RPE differentiation with a simple, scalable workflow

- Generate immature RPE cells from hPSCs in only 14 days

- Differentiate highly pure, functional RPE cells validated across multiple human ESC and iPSC lines

- Gain confidence with a convenient, user-friendly, and scalable workflow

Catalog #: 100-1367

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.