Overview

Use this optimized three-step workflow to reproducibly generate B cells and antibody-secreting cells (ASCs) from cord blood-derived CD34+ hematopoietic stem and progenitor cells (HSPCs). StemSpan™ B Cell Generation Kit provides a serum- and feeder-free protocol to reduce variation and promotes a high frequency and yield of B cells and IgM ASCs.

StemSpan™ B Cell Differentiation Supplement 1 (20X) contains a combination of recombinant cytokines and other additives formulated to selectively promote the expansion and differentiation of CD34+ cells isolated from cord blood to B lymphoid progenitor cells. Continuing the workflow, StemSpan™ B Cell Differentiation Supplement 2 (20X) provides additional stimuli to enable the further differentiation of B lymphoid progenitor cells to B cell progenitors. Finally, using StemSpan™ B Cell Differentiation Supplement 3 (20X) in tandem with StemSpan™ B Cell Differentiation Supplement 4 (20X) selectively differentiates the B cell progenitors to B cells and ASCs.

StemSpan™ B Cell Generation Kit is intended for use in combination with StemSpan™ Serum-Free Expansion Medium II (SFEM II). For your convenience, all kit components are available for purchase individually.

Subtype
Complete Kits, Supplements
 

Cell Type
B Cells
 

Species
Human
 

Application
Cell Culture, Differentiation, Expansion
 

Brand
StemSpan
 

Area of Interest
Immunology
 

Formulation Category
Serum-Free

Product Advantages

- Achieve a high yield and frequency of CD19+ B cells and antibody-secreting cells from CD34+ HSPCs

- Obtain functional B cells capable of secreting antibodies

- Generate B cells consistently without the use of serum, feeder cells, or coating materials

What's Included

- StemSpan™ B Cell Differentiation Supplement 1 (20X), 2.5 mL

- StemSpan™ B Cell Differentiation Supplement 2 (20X), 2.5 mL

- StemSpan™ B Cell Differentiation Supplement 3 (20X), 0.625 mL

- StemSpan™ B Cell Differentiation Supplement 4 (20X), 0.625 mL

- StemSpan™ SFEM II, 2 x 100 mL

Catalog #: 100-1250

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.