Overview

StemSpan™ CD34+ Expansion Supplement (10X) contains a combination of recombinant human cytokines and other additives formulated to selectively promote the expansion of CD34+ cells isolated from human cord blood (CB) or bone marrow (BM) samples.

StemSpan™ CD34+ Expansion Supplement typically promotes ~40-fold expansion of total nucleated cells in 7-day liquid cultures of CD34+ human cord blood (CB) cells. After one week, ~40% of the cultured cells express CD34, indicative of >10-fold expansion of input CD34+ CB cells. This expansion may be further increased with the addition of small molecules such as UM729. See data tab for more details.

StemSpan™ CD34+ Expansion Supplement (10X) is intended for use in combination with any of the following StemSpan™ media:
- StemSpan™ SFEM (Catalog #09600)
- StemSpan™ SFEM II (Catalog #09605)
- StemSpan™-XF (Catalog #100-0073)
- StemSpan™-AOF (Catalog #100-0130)

Contains
- Recombinant human fms-like tyrosine kinase 3 ligand (Flt3L)
- Recombinant human stem cell factor (SCF)
- Recombinant human interleukin 3 (IL-3)
- Recombinant human interleukin 6 (IL-6)
- Recombinant human thrombopoietin (TPO)
- Other additives
 

Subtype
Supplements
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human
 

Application
Cell Culture, Expansion
 

Brand
StemSpan
 

Area of Interest
Stem Cell Biology, Transplantation Research
 

Formulation Category
Serum-Free

Product Advantages

- Formulated to selectively expand and produce large numbers of human CD34+ hematopoietic cells in liquid cultures initiated with CD34+ CB or BM cells.
- Optimized for use with StemSpan™ media. When combined with StemSpan™ SFEM II in particular, supports at least 50% higher expansion of CD34+ human CB cells when compared to other serum-free media on the market.
- Supplied as a 10X concentrate. After thawing and mixing, the tube contents can be added directly to any hematopoietic cell expansion medium of choice.

Catalog #: 02691

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.