Overview

StemSpan™ Serum-Free Expansion Medium (SFEM) has been developed and tested for the in vitro culture and expansion of human hematopoietic cells, when the appropriate growth factors and supplements are added. This allows users the flexibility to prepare medium that meets their requirements. When combined with the appropriate cytokines, SFEM has been used for the culture and expansion of hematopoietic cells isolated from other species, including mouse, non-human primate, and dog. SFEM has also been used for culture of various other hematopoietic and non-hematopoietic cell types. Using appropriate StemSpan™ Expansion Supplements, SFEM may be used to expand CD34+ cells isolated from human cord blood, mobilized peripheral blood, or bone marrow samples, or to expand and differentiate lineage-committed progenitors to generate populations of erythroid, myeloid, or megakaryocyte progenitor cells.

StemSpan™ SFEM II (Catalog #09605) is an improved version of StemSpan™ SFEM that is further enriched to promote and support higher rates of CD34+ expansion and/or cell differentiation.

Contains
- Iscove’s MDM
- Bovine serum albumin
- Recombinant human insulin
- Human transferrin (iron-saturated)
- 2-Mercaptoethanol
- Supplements
 

Subtype
Specialized Media
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human, Mouse, Non-Human Primate, Rat
 

Application
Cell Culture, Expansion
 

Brand
StemSpan
 

Area of Interest
Stem Cell Biology, Transplantation Research
 

Formulation Category
Serum-Free

Catalog #: 09600 (100 mL), 09650 (500 mL)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.