Overview

StemSpan™ Serum-Free Expansion Medium II (SFEM II) is a modified version of StemSpan™ SFEM. It has been developed for the in vitro culture and expansion of human hematopoietic cells. This medium contains pre-tested bovine serum albumin, insulin, transferrin, and other supplements in Iscove’s MDM. Recombinant hematopoietic growth factors, required for the optimal growth and expansion of hematopoietic cells, have not been added to StemSpan™ SFEM II. This allows users the flexibility to prepare medium that meets their requirements.

Using appropriate cytokines (e.g. StemSpan™ CC100, StemSpan™ CC110, or StemSpan™ CD34+ Expansion Supplement), StemSpan™ SFEM II can be used for the expansion of total nucleated cells and CD34+ cells from cord blood, bone marrow, or other cell sources. StemSpan™ SFEM II can also be used to expand and differentiate lineage-committed progenitor cells to generate erythroblasts, granulocytes, monocytes, or megakaryocytes when used with StemSpan™ Erythroid Expansion Supplement (Catalog #02692), StemSpan™ Myeloid Expansion Supplement (Catalog #02693), StemSpan™ Myeloid Expansion Supplement II (Catalog #02694), or StemSpan™ Megakaryocyte Expansion Supplement (Catalog #02696), respectively.

Contains
- Iscove’s MDM
- Bovine serum albumin
- Recombinant human insulin
- Human transferrin (iron-saturated)
- 2-Mercaptoethanol
- Supplements
 

Subtype
Specialized Media
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human
 

Application
Cell Culture, Expansion
 

Brand
StemSpan
 

Area of Interest
Stem Cell Biology, Transplantation Research
 

Formulation Category
Serum-Free

Catalog #: 09605 (100 mL), 09655 (500 mL)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.