Overview

Use this two-step protocol to reproducibly expand thousands of CD4⁺CD8⁺ double-positive (DP) T cells in cultures initiated with CD34⁺ human cord blood (CB) cells.

In step one, CD34⁺ CB cells are cultured for 14 days in medium containing expansion supplement to promote their proliferation and differentiation into CD7⁺CD5⁺ pro-T cells. In step two, pro-T cells generated during those first 14 days are cultured for another 4 weeks in medium containing maturation supplement to promote their maturation into DP cells. This medium kit is serum- and feeder-free, eliminating variation introduced by serum and stromal cell lines.

For your convenience, all kit components are also available for purchase individually. StemSpan™ Lymphoid Progenitor Expansion Supplement (10X) contains a combination of recombinant human cytokines and other additives formulated to selectively promote the expansion and differentiation of CD34⁺ cells isolated from human CB samples to CD7⁺CD5⁺ progenitor T (pro-T) cells. Additionally, StemSpan™ T Cell Progenitor Maturation Supplement (10X) enables further maturation of pro-T cells to DP cells. It also supports the maturation of DP cells to CD8 single-positive (SP) T cells with additional stimuli. StemSpan™ Lymphoid Progenitor Expansion Supplement (10X) and StemSpan™ T Cell Progenitor Maturation Supplement (10X) are intended for use in combination with StemSpan™ SFEM II medium, and on plates coated with StemSpan™ Lymphoid Differentiation Coating Material (100X).

An optional protocol is available to further mature DP cells to CD8 SP T cells. This extended protocol uses a complete T cell progenitor maturation medium made with reagents included in the StemSpan™ T Cell Generation Kit and must be combined with ImmunoCult™ CD3/CD28/CD2 T Cell Activator and IL-15.

Subtype
Specialized Media, Supplements
 

Cell Type
Hematopoietic Stem and Progenitor Cells, T Cells
 

Species
Human
 

Application
Cell Culture, Differentiation, Expansion
 

Brand
StemSpan
 

Area of Interest
Cancer, Immunology, Stem Cell Biology, Cell Therapy Development
 

Formulation Category
Serum-Free

What's Included

- StemSpan™ SFEM II, 2 x 100 mL

- StemSpan™ Lymphoid Progenitor Expansion Supplement (10X), 5 mL

- StemSpan™ Lymphoid Differentiation Coating Material (100X), 2 x 0.25 mL

- StemSpan™ T Cell Progenitor Maturation Supplement (10X), 12.5 mL

Catalog #: 09940

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.