Overview

StemSpan™-AOF is an animal origin-free (AOF) medium that has been developed for the in vitro culture and expansion of human hematopoietic cells, when appropriate growth factors/cytokines and supplements (e.g. small molecules) are added. This allows users the flexibility to prepare medium that meets their requirements.

StemSpan™-AOF contains only recombinant proteins and synthetic components, and does not contain serum or other human- or animal-derived components.

StemSpan™-AOF has also demonstrated a higher capacity than other commercially available media for the expansion of both CD34⁺CD90⁺CD45RA⁻ and CD34brightCD90⁺CD45RA⁻ subsets, enriched for hematopoietic stem cells: CD34+ cells from cord blood expanded in StemSpan™-AOF for 7 days demonstrated similar or higher repopulation than uncultured cells when transplanted into sub-lethally irradiated NSG mice and measured at both short- (3 week) and long-term (20 week) engraftment timepoints.

StemSpan™-AOF (Catalog #100-0130) is manufactured under relevant cGMPs, ensuring the highest quality and consistency for reproducible results. For additional quality information, visit www.STEMCELL.com/compliance.

STEMCELL TECHNOLOGIES maintains registered Drug Master Files for StemSpan™-AOF with the US Food and Drug Administration (FDA) and with the Japan Pharmaceuticals and Medical Devices Agency (PMDA). Request a Letter of Authorization (LOA) for StemSpan™-AOF's Drug Master File.

Note: The FDA Master File is in the process of being updated with the new format of StemSpan™-AOF: currently only the 500ml format (Catalog #100-0130) is referenced.

Please note, StemSpan™-AOF was originally launched as StemSpan™-ACF Without Phenol Red. This name change signifies that in addition to being animal component-free, no materials of animal or human origin are used in the manufacture of this medium or its components, to at least the secondary level of manufacturing. This medium also replaces StemSpan™-ACF (Catalog #09855).

Contains
- Phenol Red-Free Iscove’s MDM
- Recombinant human albumin
- Recombinant human insulin
- Recombinant human transferrin
- 2-Mercaptoethanol
- Supplements
 

Subtype
Specialized Media
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human
 

Application
Cell Culture, Differentiation, Expansion, Genome Editing
 

Brand
StemSpan
 

Area of Interest
Stem Cell Biology, Transplantation Research, Cell Therapy Development
 

Formulation Category
Animal Origin-Free, Serum-Free, Xeno-Free

Product Advantages

- 20X expansion of human cord blood-derived CD34+ cells after 7 days of culture

- Maintenance of primitive CD34^brightCD90+CD45RA- population in culture

- Suitable for use in genome editing protocols

- Animal origin- and phenol red-free formulation

- Manufactured and tested under relevant cGMPs

- Full traceability of raw materials

Catalog #: 100-0130 (500 mL Bottle), 100-0513 (1 L Bag)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.