Overview

The Complete Kit for Human Whole Blood CD34+ Cells is a simple two-step method that is designed to isolate CD34+ cells from whole blood or buffy coat.

First, hematopoietic progenitor cells are pre-enriched using the RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail (15186C) with antibodies recognizing CD2, CD3, CD14, CD16, CD19, CD24, CD56, CD66b and CD61 surface markers. CD34+ cells are then selected using the EasySep™ Human CD34 Positive Selection Cocktail (18066C), which contains an antibody recognizing CD34. RosetteSep™ binds unwanted cells to red blood cells (RBCs), forming immunorosettes, which sediment during density gradient centrifugation. The pre-enriched fraction containing CD34+ cells is harvested from the interface between the plasma and density gradient medium. The pre-enriched CD34+ cells are then labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated CD34+ cells are immediately available for downstream applications.

Magnet Compatibility
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Hematopoietic Stem and Progenitor Cells
 

Species
Human
 

Sample Source
Buffy Coat, Whole Blood
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep, RosetteSep
 

Area of Interest
Immunology, Stem Cell Biology

Product Advantages

- Fast and easy-to-use

- Up to 98% purity

- No columns required

- Can be combined with SepMate™ for consistent, high-throughput sample processing

What's Included

- Complete Kit for Human Whole Blood CD34+ Cells (Catalog #15086)

    - RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail, 3 x 2 mL

    - EasySep™ Human CD34 Positive Selection Cocktail, 0.4 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

- RoboSep™ Complete Kit for Human WB CD34 (Catalog #15086RF)

    - RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail, 3 x 2 mL

    - EasySep™ Human CD34 Positive Selection Cocktail, 0.4 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 15086 (EasySep + RosetteSep), 15086RF (RosetteSep + RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.