Overview

Isolate CD4+CD304+ regulatory T cells (Tregs) from single-cell suspensions of mouse splenocytes or lymph nodes, in less than 45 minutes, using column-free immunomagnetic positive selection. First, CD304+ cells are isolated using EasySep™ Release technology. Then, bound magnetic particles are removed from the EasySep™-isolated CD304+ cells, and unwanted non-Tregs are targeted for depletion. The final isolated fraction contains highly purified CD4+CD304+ cells that express high levels of FOXP3 and CD25 Treg markers and are immediately ready for downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction. An optional protocol allows for the isolation of CD4+CD304- responder T cells in parallel for use in functional studies.

This kit is a first-of-its-kind mouse Treg isolation kit that targets the CD304 surface marker, which, unlike the CD25 Treg marker commonly used for Treg isolation, does not interfere with IL-2 signaling. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.

Magnet Compatibility
EasySep™ Magnet (Catalog #18000)
“The Big Easy” EasySep™ Magnet (Catalog #18001)
EasyEights™ EasySep™ Magnet (Catalog #18103)
RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
T Cells, T Cells, Regulatory
 

Species
Mouse
 

Sample Source
Lymph Node, Other, Spleen
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Cancer, Immunology, Infectious Diseases

Product Advantages

- Obtain up to 94% cell purity in under 45 minutes

- Yield particle-free CD25+ Tregs and prevent interference with IL-2 signaling

- Avoid time-consuming wash steps with EasySep™ Releasable RapidSpheres™

- Isolate CD4+CD304- responder T cells from the same sample if needed

What's Included

- EasySep™ Release Mouse CD4+CD304+ Regulatory T Cell Isolation Kit (Catalog #100-1570)

    - EasySep™ Mouse CD4+ T Cell Isolation Cocktail, 1 x 0.5 mL

    - EasySep™ Mouse CD304 Positive Selection Cocktail, 1 x 0.5 mL

    - EasySep™ Release PE Positive Selection Cocktail, 1 x 0.5 mL

    - EasySep™ Releasable RapidSpheres™ 50201, 1 x 1 mL

    - EasySep™ Streptavidin RapidSpheres™ 50001, 1 x 1 mL

    - EasySep™ Release Buffer (Concentrate), 3 x 1 mL

    - EasySep™ Mouse FcR Blocker, 1 x 0.2 mL

- RoboSep™ Release Mouse CD4+CD304+ Regulatory T Cell Isolation Kit (Catalog #100-1564)

    - EasySep™ Mouse CD4+ T Cell Isolation Cocktail, 1 x 0.5 mL

    - EasySep™ Mouse CD304 Positive Selection Cocktail, 1 x 0.5 mL

    - EasySep™ Release PE Positive Selection Cocktail, 1 x 0.5 mL

    - EasySep™ Releasable RapidSpheres™ 50201, 1 x 1 mL

    - EasySep™ Streptavidin RapidSpheres™ 50001, 1 x 1 mL

    - EasySep™ Release Buffer (Concentrate), 3 x 1 mL

    - EasySep™ Mouse FcR Blocker, 1 x 0.2 mL

    - RoboSep™ Filter Tips (Catalog #20125) x 2

    - RoboSep™ Buffer (Catalog #20104)

    - EasySep™ EasyTube-14 (Catalog #20128)

Catalog #: 100-1570 (EasySep), 100-1564 (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.