Overview

Efficiently deplete apoptotic (Annexin V+) cells from cell culture or tissue preparation samples by immunomagnetic negative selection, with the EasySep™ Dead Cell Removal (Annexin V) Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

This straightforward, optimized EasySep™ procedure involves labeling cells with antibody complexes recognizing Annexin V and magnetic particles. Labeled cells are separated from untouched cells using an EasySep™ magnet and by simply pouring or pipetting off the unlabeled cells. The Annexin V+ cells remain in the tube. Following magnetic cell isolation, desired cells are ready for downstream applications. Annexin V binds phosphatidylserine on the outer leaflet of the cell membrane during apoptosis.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000), or
- “The Big Easy” EasySep™ Magnet (Catalog #18001), or
- EasyEights™ EasySep™ Magnet (Catalog #18103)
 
Subtype
Cell Isolation Kits
 

Cell Type
Lymphocytes
 

Species
Human, Mouse, Non-Human Primate, Other, Rat
 

Sample Source
Cord Blood, Leukapheresis, Lung, Lymph Node, Other, Spleen
 

Selection Method
Depletion
 

Brand
EasySep
 

Area of Interest
Immunology

Product Advantages

- Fast and easy-to-use

- No columns required

- Compatible across EasySep™, "The Big Easy", and EasyEights™ platforms

What's Included

- EasySep™ Dead Cell Removal (Annexin V) Kit (Catalog #17899)

    - EasySep™ Dead Cell Removal (Annexin V) Cocktail, 0.5 mL

    - EasySep ™ Biotin Selection Cocktail, 1 mL

    - EasySep™ Dextran RapidSpheres™ 50103, 1 mL

Catalog #: 17899

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.