Overview

Easily and efficiently isolate highly purified human neutrophils directly from human whole blood samples by immunomagnetic negative selection, with the EasySep™ Direct Human Neutrophil Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles called EasySep™ Direct RapidSpheres™. The following unwanted cells are targeted for removal: basophils, eosinophils, T cells, B cells, monocytes, NK cells, and erythroid cells. The magnetically labeled cells are then separated from the untouched desired neutrophils by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired neutrophils are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This product replaces the EasySep™ Human Neutrophil Isolation Kit (Catalog #19257) for even faster cell isolations.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Granulocytes and Subsets
 

Species
Human
 

Sample Source
Whole Blood
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology, Infectious Diseases

Product Advantages

- > 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation, or lysis

- Up to 99% purity of isolated cells

- Fast, easy-to-use and column-free

- Isolated cells are untouched

What's Included

- EasySep™ Direct Human Neutrophil Isolation Kit (Catalog #19666)

    - EasySep™ Direct Human Neutrophil Isolation Cocktail, 2 x 2.5 mL

    - EasySep™ Direct RapidSpheres™, 4 x 2.5 mL

- RoboSep™ Human Neutrophil Isolation Kit (Catalog #100-0404)

    - EasySep™ Direct Human Neutrophil Isolation Cocktail, 2 x 2.5 mL

    - EasySep™ Direct RapidSpheres™, 4 x 2.5 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19666 (EasySep), 100-0404 (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.