Overview

Easily and efficiently isolate highly purified human peripheral blood mononuclear cells (PBMCs) from fresh whole blood, buffy coat, bone marrow, cord blood, or leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Direct Human PBMC Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles called EasySep™ Direct RapidSpheres™. The following unwanted cells are targeted for removal: granulocytes, platelets, and RBCs. The magnetically labeled cells are then separated from the untouched desired PBMCs by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired PBMCs are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- Easy 50 EasySep™ Magnet (Catalog #18002)
 

Subtype
Cell Isolation Kits
 

Cell Type
B Cells, Lymphocytes, Monocytes, Mononuclear Cells, NK Cells, T Cells
 

Species
Human
 

Sample Source
Bone Marrow, Buffy Coat, Cord Blood, Leukapheresis, Whole Blood
 

Selection Method
Negative
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation or lysis

- Fast, easy-to-use and column-free

- Isolated cells are untouched

What's Included

- EasySep™ Direct Human PBMC Isolation Kit (Catalog #19654)

    - EasySep™ Direct Human PBMC Isolation Cocktail, 2 x 2.5 mL

    - EasySep™ Direct RapidSpheres™, 4 x 2.5 mL

- EasySep™ Direct Human PBMC Isolation Kit for RoboSep™ (Catalog #19654RF)

    - EasySep™ Direct Human PBMC Isolation Cocktail, 2 x 2.5 mL

    - EasySep™ Direct RapidSpheres™, 4 x 2.5 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19654 (EasySep), 19654RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.