Overview

Easily and efficiently isolate highly purified human total lymphocytes from human whole blood, buffy coat, or spleen samples by immunomagnetic negative selection, with the EasySep™ Direct Human Total Lymphocyte Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles called EasySep™ Direct RapidSpheres™. The following unwanted cells are targeted for removal: monocytes, neutrophils, eosinophils, and platelets. The magnetically labeled cells are then separated from the untouched desired human total lymphocytes by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired total lymphoctes are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Lymphocytes
 

Species
Human
 

Sample Source
Whole Blood
 

Selection Method
Negative
 

Application
Cell Isolation
 

Brand
EasySep
 

Area of Interest
Chimerism, HLA, Immunology

Product Advantages

- > 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation or lysis

- Fast, easy-to-use and column-free

- Up to 96% purity of isolated cells

- Isolated cells are untouched

What's Included

- EasySep™ Direct Human Total Lymphocyte Isolation Kit (Catalog #19655)

    - EasySep™ Direct Human Total Lymphocyte Isolation Cocktail, 2 x 2.5 mL

    - EasySep™ Direct RapidSpheres™, 4 x 2.5 mL

- EasySep™ Direct Human Total Lymphocyte Isolation Kit for RoboSep™ (Catalog #19655RF)

    - EasySep™ Direct Human Total Lymphocyte Isolation Cocktail, 2 x 2.5 mL

    - EasySep™ Direct RapidSpheres™, 4 x 2.5 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 19655 (EasySep), 19655RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.