Overview

The EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit is designed to isolate B cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19, CD20 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is present on mature B cells and B cell progenitors, but is lost upon maturation to plasma cells. The antigen CD20 is expressed on all stages of B cell development except on pro B cells or plasma cells.

This product replaces the EasySep™ HLA Whole Blood B Cell Positive Selection Kit (Catalog #18184HLA) for even faster cell isolations.

Subtype
Cell Isolation Kits
 

Cell Type
B Cells
 

Species
Human
 

Sample Source
Buffy Coat, Peripheral Blood
 

Selection Method
Positive
 

Brand
EasySep, RoboSep
 

Area of Interest
Chimerism, HLA, Immunology

Product Advantages

- Fast and easy-to-use

- Up to 97% purity

- No columns required

What's Included

- EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit (Catalog #17886)

    - EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Cocktail, 3 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL

    - EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)

- RoboSep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit (Catalog #17886RF)

    - EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Cocktail, 3 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL

    - EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)

    - RoboSep™ Buffer (Catalog #20104) x 2

    - RoboSep™ Filter Tips (Catalog #20125) x 2

Catalog #: 17886 (EasySep), 17886RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.