Overview

The EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit is designed to isolate CD19+ cells from whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is present on mature B cells and B cell progenitors but is lost upon maturation to plasma cells. It is also expressed on follicular dendritic cells.

This product replaces the EasySep™ Human Whole Blood CD19 Positive Selection Kit (Catalog #18084) for even faster cell isolations.

Magnet Compatibility
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
B Cells
 

Species
Human
 

Sample Source
Buffy Coat, Whole Blood
 

Selection Method
Positive
 

Brand
EasySep
 

Area of Interest
HLA, Immunology

Product Advantages

- Fast and easy-to-use

- Up to 99% purity

- No columns required

- Compatible across EasySep™ "The Big Easy", EasyEights™, and RoboSep™-S platforms

What's Included

- EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit (Catalog #17874)

    - EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Cocktail, 3 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL

    - EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)

- RoboSep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit (Catalog #17874RF)

    - EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Cocktail, 3 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL

    - EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)

    - RoboSep™ Buffer (Catalog #20104) x 2

    - RoboSep™ Filter Tips (Catalog #20125) x 2

Catalog #: 17874 (EasySep), 17874RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.