Overview

Isolate highly purified human CD3+ cells from fresh whole blood or buffy coat samples by immunomagnetic positive selection, with the EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD3 and magnetic particles. The cocktail in this kit also contains an antibody to human Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD3+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This product replaces the EasySep™ Human Whole Blood CD3 Positive Selection Kit (Catalog #18081) and EasySep™ HLA Whole Blood CD3 Positive Selection Kit (Catalog #18081HLA), for even faster cell isolations.

Magnet Compatibility
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 
Subtype
Cell Isolation Kits
 

Cell Type
T Cells
 

Species
Human
 

Sample Source
Buffy Coat, Whole Blood
 

Selection Method
Positive
 

Brand
EasySep
 

Area of Interest
HLA, Immunology

Product Advantages

- Fast and easy-to-use

- Up to 99% purity

- No columns required

- Compatible across EasySep™ "The Big Easy", EasyEights™, and RoboSep™-S platforms

What's Included

- EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit (Catalog #17871)

    - EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Cocktail, 3 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL

    - EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)

- RoboSep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit with Filter Tips (Catalog #17871RF)

    - EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Cocktail, 3 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL

    - EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)

    - RoboSep™ Buffer (Catalog #20104) x 2

    - RoboSep™ Filter Tips (Catalog #20125) x 2

Catalog #: 17871 (EasySep), 17871RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.