Overview

The EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit is designed to isolate CD8+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD8 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD8 antigen is expressed on cytotoxic T cells and weakly on a subset of NK cells.

This product replaces the EasySep™ Human Whole Blood CD8 Positive Selection Kit (Catalog #18083) for even faster cell isolations.

Magnet Compatibility
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 
Subtype
Cell Isolation Kits
 

Cell Type
T Cells, CD8+
 

Species
Human
 

Sample Source
Buffy Coat, Whole Blood
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Chimerism, HLA, Immunology

Product Advantages

- Fast and easy-to-use

- Up to 99.9% purity

- No columns required

What's Included

- EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit (Catalog #17889)

    - EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Cocktail, 3 x 1 mL

    - EasySep™ Dextran RapidSpheres™ (Catalog #50101), 3 x 1 mL

    - EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)

- RoboSep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit with Filter Tips (Catalog #17889RF)

    - EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Cocktail, 3 x 1 mL

    - EasySep™ Dextran RapidSpheres™ (Catalog #50101), 3 x 1 mL

    - EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)

    - RoboSep™ Buffer (Catalog #20104) x 2

    - RoboSep™ Filter Tips (Catalog #20125) x 2

Catalog #: 17889 (EasySep), 17889RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.