Overview

The EasySep™ Human Whole Blood Lymphoid Positive Selection Kit is designed to isolate CD3+ and CD19+ cells from whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3, CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T lymphocytes and NKT cells while the CD19 antigen is expressed on B cells and follicular dendritic cells.

This product replaces the EasySep™ Human Whole Blood Lymphoid Positive Selection Kit (Catalog #18684) and EasySep™ HLA Whole Blood Lymphoid Positive Selection Kit (Catalog #18684HLA) for even faster cell isolations.

Magnet Compatibility
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
B Cells, Lymphocytes, T Cells
 

Species
Human
 

Sample Source
Buffy Coat, Peripheral Blood, Whole Blood
 

Selection Method
Positive
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast and easy-to-use

- Up to 99.9% purity

- No columns required

What's Included

- EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit (Catalog #17873)

    - EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Cocktail, 3 x 1 mL

    - EasySep™ Dextran RapidSpheres 50101, 3 x 1 mL

    - EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)

- RoboSep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit (Catalog #17873RF)

    - EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Cocktail, 3 x 1 mL

    - EasySep™ Dextran RapidSpheres 50101, 3 x 1 mL

    - EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)

    - RoboSep™ Buffer (Catalog #20104) x 2

    - RoboSep™ Filter Tips (Catalog #20125) x 2

Catalog #: 17873 (EasySep), 17873RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.