Overview

Isolate highly purified human CD33+ and CD66b+ myeloid cells, including myeloid progenitor cells, granulocytes, and monocytes, from fresh human whole blood or buffy coat samples by immunomagnetic positive selection, with the EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD33, CD66b, and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD33+ and CD66+ myeloid cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction for lineage-specific chimerism analysis.

This product has replaced the EasySep™ Human Whole Blood Myeloid Positive Selection Kit (Catalog #18683) and EasySep™ HLA Whole Blood Myeloid Positive Selection Kit (Catalog #18683HLA) for faster cell isolations.

Magnet Compatibility
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Centrifugation Tubes
 

Cell Type
Myeloid Cells
 

Species
Human
 

Sample Source
Whole Blood
 

Selection Method
Positive
 

Brand
EasySep
 

Area of Interest
Chimerism, Immunology

Product Advantages

- Fast and easy-to-use

- Up to 99% purity

- No columns required

What's Included

- EasySepSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit (Catalog #17884)

    - EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Cocktail, 3 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL

    - EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)

- RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit with Filter Tips (Catalog #17884RF)

    - EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Cocktail, 3 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL

    - EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)

    - RoboSep™ Buffer (Catalog #20104) x 2

    - RoboSep™ Filter Tips (Catalog #20125) x 2

Catalog #: 17884 (EasySep), 17884RF (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.