Overview

Efficiently isolate or deplete human CD11b+ cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples in as little as 16 minutes. Cells isolated with the EasySep™ Human CD11b Positive Selection and Depletion Kit can be used immediately in downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction.

Use this kit to label CD11b+ cells with antibodies and magnetic particles. The cells are then subsequently separated—without the use of columns—using an EasySep™ magnet. Labeled cells remain in the tube while unlabeled cells are poured off. Depending on your research needs, either fraction can be retained for subsequent applications.

This kit targets human CD11b, a subunit of the integrin macrophage-1 antigen (Mac-1) receptor commonly used as a myeloid cell marker. CD11b is expressed on immune cells such as monocytes, macrophages, neutrophils, and NK cell subsets, and is known to play a role in regulating cell adhesion, migration, and phagocytosis (Khan SQ et al. Front Med, 2018).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
Granulocytes and Subsets, Macrophages, Monocytes, NK Cells
 

Species
Human
 

Sample Source
Leukapheresis, Peripheral Blood
 

Selection Method
Depletion, Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology, Infectious Diseases

Product Advantages

- Fast and easy-to-use

- Up to 99% purity

- No columns required

What's Included

- EasySep™ Human CD11b Positive Selection and Depletion Kit (Catalog #100-0742)

    - EasySep™ Human CD11b Positive Selection Depletion Cocktail, 0.75 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL

- RoboSep™ Human CD11b Positive Selection and Depletion Kit (Catalog #100-0744)

    - EasySep™ Human CD11b Positive Selection Depletion Cocktail, 0.75 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL

    - RoboSep™ Buffer (Catalog #20104)

   - RoboSep™ Tips (Catalog #20125)

Catalog #: 100-0742 (EasySep), 100-0744 (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.