Overview

Isolate highly purified human CD138+ (syndecan-1) cells from fresh or previously frozen human bone marrow or peripheral blood mononuclear cell (MNC) samples by immunomagnetic positive selection, with the EasySep™ Human CD138 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD138 and magnetic particles. The cocktail in this kit also contains an antibody to human Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD138+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The CD138 antigen is expressed on normal and malignant plasma cells (but not mature B cells).

This product replaces the EasySep™ Human CD138 Positive Selection Kit (Catalog #18357) for even faster cell isolations.

If isolating CD138+ cells from whole blood and bone marrow, the EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit (Catalog #17887) is recommended.

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
 

Subtype
Cell Isolation Kits
 

Cell Type
B Cells, Plasma
 

Species
Human
 

Sample Source
Bone Marrow, PBMC
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Cancer, Immunology

Product Advantages

- Fast and easy-to-use

- No columns required

What's Included

- EasySep™ Human CD138 Positive Selection Kit II (Catalog #17877)

    - EasySep™ Human CD138 Positive Selection Kit II Cocktail, 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

- RoboSep™ Human CD138 Positive Selection Kit II with Filter Tips (Catalog #17877RF)

   - EasySep™ Human CD138 Positive Selection Kit II Cocktail, 1 mL

   - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

   - RoboSep™ Buffer (Catalog #20104)

   - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 17877 (EasySep), 17877 (RoboSep)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.