Overview

Isolate highly purified human CD3+ cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples by immunomagnetic positive selection, with the EasySep™ Human CD3 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD3 and magnetic particles. The cocktail in this kit also contains an antibody to human Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation in as little as 15 minutes, the desired CD3+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This product replaces the EasySep™ Human CD3 Positive Selection Kit (Catalog #18051) for even faster cell isolations.

For large-scale isolation of human CD3+ cells from leukapheresis samples, see the large-format (1x10¹⁰ cells) kit (Catalog #100-0692).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- EasyPlate™ EasySep™ Magnet (Catalog #18102)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- RoboSep™-S (Catalog #21000)
- Easy 250 EasySep™ Magnet (Catalog #100-0821)
 

Subtype
Cell Isolation Kits
 

Cell Type
T Cells
 

Species
Human
 

Sample Source
PBMC
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Chimerism, Immunology, Cell Therapy Development

Product Advantages

- Fast and easy-to-use

- Up to 99% purity

- No columns required

What's Included

- EasySep™ Human CD3 Positive Selection Kit II (Catalog #17851)

    - EasySep™ Human CD3 Positive Selection Cocktail II, 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

- EasySep™ Human CD3 Positive Selection Kit II (Catalog #100-0692)

    - EasySep™ Human CD3 Positive Selection Cocktail II, 1 x 10 mL

    - EasySep™ Dextran RapidSpheres™ 50103, 2 x 1 mL

- RoboSep™ Human CD3 Positive Selection Kit II (Catalog #17851RF)

    - EasySep™ Human CD3 Positive Selection Cocktail II, 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 17851 (EasySep 1 x 10^9 cells), 17851RF (RoboSep 1 x 10^9 cells), 100-0692 (EasySep 1 x 10^10 cells)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.