Overview

Isolate highly purified human CD34+ cells from fresh or previously frozen mobilized human peripheral blood or bone marrow mononuclear cells (MNCs), previously frozen cord blood MNCs, or human embryonic stem (ES) and induced pluripotent stem (iPS) cell cultures by immunomagnetic positive selection, with the EasySep™ Human CD34 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD34 and magnetic particles. The cocktail in this kit also contains an antibody to human Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep™ magnet and by simply pouring off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired human CD34+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.

This product replaces the EasySep™ Human CD34 Positive Selection Kit (Catalog #18056) for even faster cell isolations.

For large-scale isolation of human CD34+ cells from mobilized leukopaks, see the large-format (1x10¹⁰ cells) kit (Catalog #100-1569).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- RoboSep™-S (Catalog #21000)
- Easy 250 EasySep™ Magnet (Catalog #100-0821)
 

Subtype
Cell Isolation Kits
 

Cell Type
Hematopoietic Stem and Progenitor Cells, Pluripotent Stem Cells
 

Species
Human
 

Sample Source
Bone Marrow, Cord Blood, Other, PBMC, Pluripotent Stem Cells, Whole Blood, Mobilized Leukopaks
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Chimerism, Immunology, Stem Cell Biology

Product Advantages

- Fast and easy-to-use

- Up to 99% purity

- No columns required

What's Included

- EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856)

    - EasySep™ Human CD34 Positive Selection Cocktail, 1 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 x 1 mL

- EasySep™ Human CD34 Positive Selection Kit II (Catalog #100-1569)

    - EasySep™ Human CD34 Positive Selection Cocktail, 1 x 10 mL

    - EasySep™ Dextran RapidSpheres™ 50103, 1 x 1 mL

- RoboSep™ Human CD34 Positive Selection Kit II with Filter Tips (Catalog #17856RF)

    - EasySep™ Human CD34 Positive Selection Cocktail, 1 x 1 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 x 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 17856 (EasySep 5 x 10^9 cells), 17856RF (RoboSep 5 x 10^9 cells), 100-1569 (EasySep 1 x 10^10 cells)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.