Overview

Isolate highly purified human CD4+ cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples by immunomagnetic positive selection, with the EasySep™ Human CD4 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD4 and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation in as little as 15 minutes, the desired CD4+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This product replaces the EasySep™ Human CD4 Positive Selection Kit (Catalog #18052) for even faster cell isolations.

For large-scale isolation of CD4+ cells from leukapheresis samples, see the large-format (1x10¹⁰ cells) kit (Catalog #100-0693).

Magnet Compatibility
- EasySep™ Magnet (Catalog #18000)
- “The Big Easy” EasySep™ Magnet (Catalog #18001)
- Easy 50 EasySep™ Magnet (Catalog #18002)
- EasyEights™ EasySep™ Magnet (Catalog #18103)
- RoboSep™-S (Catalog #21000)
- Easy 250 EasySep™ Magnet (Catalog #100-0821)
 

Subtype
Cell Isolation Kits
 

Cell Type
T Cells, T Cells, CD4+
 

Species
Human
 

Sample Source
Leukapheresis, PBMC
 

Selection Method
Positive
 

Application
Cell Isolation
 

Brand
EasySep, RoboSep
 

Area of Interest
Immunology

Product Advantages

- Fast and easy-to-use

- Up to 96% purity

- No columns required

What's Included

- EasySep™ Human CD4 Positive Selection Kit II (Catalog #17852)

    - EasySep™ Human CD4 Positive Selection Cocktail II, 0.5 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

- EasySep™ Human CD4 Positive Selection Kit II (Catalog #100-0693)

    - EasySep™ Human CD4 Positive Selection Cocktail II, 1 x 5 mL

    - EasySep™ Dextran RapidSpheres™ 50103, 2 x 1 mL

- RoboSep™ Human CD4 Positive Selection Kit II (Catalog #17852RF)

    - EasySep™ Human CD4 Positive Selection Cocktail II, 0.5 mL

    - EasySep™ Dextran RapidSpheres™ 50100, 1 mL

    - RoboSep™ Buffer (Catalog #20104)

    - RoboSep™ Filter Tips (Catalog #20125)

Catalog #: 17852 (EasySep 1 x 10^9 cells), 17852RF (RoboSep 1 x 10^9 cells), 100-0693 (EasySep 1 x 10^10 cells)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.